About the Podcast
mRNA is frequently encapsulated with positively charged liposomes to form lipoplexes. Lipoplexes are self-assembling nanosystems and their formation entails a multistep mechanism as revealed by detailed studies employing monolayers, atomic force microscopy and cryo-electron microscopy. In a millisecond first step, electrostatic interactions between phosphate and the positively charged amine headgroup of the cationic lipid occur. The cellular uptake is mainly mediated by either lipoplex endocytosis or endocytosis-like mechanisms. Moreover, lipoplex delivery systems have substantial advantages like easy synthesis, low batch variability, scalability, and biocompatibility. It has also been noted that drug delivery systems in the nano-range enhance the pharmacokinetic availability of encapsulated drugs. https://mrna.creative-biolabs.com/lipoplex.htm
